重組人單核小體，由大腸桿菌中表達(dá)的重組人組蛋白組裝而成(組蛋白H2A、H2B、H3和H4各2個(gè);Accession numbers:H2A-P04908;H2B-O60814;H3.1-P68431;H4- P62805)，纏繞著147個(gè)堿基對(duì)包含601序列DNA。核小體是染色質(zhì)的基本亞基。由Lowary和Widom鑒定的601序列對(duì)組蛋白八聚體具有高親和力，有利于核小體的組裝。

產(chǎn)品詳情

宿主來(lái)源: Human

表達(dá)系統(tǒng): E. coli & synthetic DNA

保存溫度: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws

運(yùn)輸溫度: Dry ice

產(chǎn)品形式: 0.84 mg/mL mononucleosome in µL 10 mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, and 20% glycerol. (54.6 µg protein, 100 µg DNA + protein)

Figure 1: DNA Gel Data Mononucleosomes, Recombinant Human run on an agarose gel and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA extracted from nucleosomes (200 ng). Lane 2: Intact nucleosomes (400 ng).

Figure 2: Protein Gel Data Coomassie stained PAGE gel of proteins in Mononucleosomes, Human Recombinant (0.5 µg) to demonstrate the purity and integrity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2B, H3 and H4) are indicated.

Figure 3: HMTase Assay Data Mononucleosomes, Recombinant Human used as a substrate in a radioactive histone methyltransferase assay with (+SET8) or without (-SET8) recombinant SET8, which is an HMTase that requires nucleosomal substrates for activity.

產(chǎn)品使用文獻(xiàn)

[1] Huang, et al. Oncohistone Mutations Occur at Functional Sites of Regulatory ADP-ribosylation. Cancer Res, 2022.

[2] Jabeena, et al. Dynamic association of the H3K64 trimethylation mark with genes encoding exported proteins in Plasmodium falciparum. JBC, 2021.

[3] Yuan, et al. Elevated NSD3 histone methylation activity drives squamous cell lung cancer. Nature, 2021.

[4] Mullahoo, et al. Dual protease type XIII/pepsin digestion offers superior resolution and overlap for the analysis of histone tails by HX-MS. Methods, 2020

[5] Yan, et al. Deficient histone H3 propionylation by BRPF1-KAT6 complexes in neurodevelopmental disorders and cancer.Science Advances, 2020.

訂購(gòu)詳情

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